https://ogma.newcastle.edu.au/vital/access/ /manager/Index en-au 5 Extracellular hydrogen peroxide, produced through a respiratory burst oxidase/superoxide dismutase pathway, directs ingrowth wall formation in epidermal transfer cells of Vicia faba cotyledons https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:21810 trans-differentiation events leading to formation of TC ingrowth walls are poorly understood. Vicia faba cotyledons offer a robust experimental model to examine TC induction as, when placed into culture, their adaxial epidermal cells rapidly (h) and synchronously form polarized ingrowth walls accessible for experimental observations. Using this model, we recently reported findings consistent with extracellular hydrogen peroxide, produced through a respiratory burst oxidase homolog/superoxide dismutase pathway, initiating cell wall biosynthetic activity and providing directional information guiding deposition of the polarized uniform wall. Our conclusions rested on observations derived from pharmacological manipulations of hydrogen peroxide production and correlative gene expression data sets. A series of additional studies were undertaken, the results of which verify that extracellular hydrogen peroxide contributes to regulating ingrowth wall formation and is generated by a respiratory burst oxidase homolog/superoxide dismutase pathway.]]> Sat 24 Mar 2018 07:59:23 AEDT ]]> Unravelling transfer cell development: wall labyrinth construction and reaction oxygen species https://ogma.newcastle.edu.au/vital/access/ /manager/Repository/uon:33230 Mon 23 Sep 2019 10:27:48 AEST ]]>